Name | 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside |
Synonyms | Xgal X-GAL X-BETA-GAL X-BETA-D-GAL X-GAL READY TO USE X-BETA-D-GALACTOSIDE 5-Bromo-4-chloro-3-indolyl-b-D-galactoside 5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactoside 5-Bromo-4-chloro-3-indolyl-.beta.-D-galactoside 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside 5-BROMO-4-CHLORO-3-INDOLYL-B B-D-GALACTOPYRANOSIDE 5-Bromo-4-chloro-1H-indol-3-yl beta-D-galactopyranoside 5-BROMO-4-CHLORO-1H-INDOL-3-YL BETA-D-GALACTOSIDE-(1,5) 5-BROMO-4-CHLORO-1H-INDOL-3-YL BETA-D-GALACTOPYRANOSIDE beta-D-Galactopyranoside, 5-bromo-4-chloro-1H-indol-3-yl .beta.-D-Galactopyranoside, 5-bromo-4-chloro-1H-indol-3-yl |
CAS | 7240-90-6 |
EINECS | 230-640-8 |
InChI | InChI=1/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1 |
InChIKey | OPIFSICVWOWJMJ-AEOCFKNESA-N |
Molecular Formula | C14H15BrClNO6 |
Molar Mass | 408.63 |
Density | 1.5563 (rough estimate) |
Melting Point | 230 °C |
Boling Point | 673.9±55.0 °C(Predicted) |
Specific Rotation(α) | -64 º (C=1, H2O/DMF (1/1)) |
Flash Point | 361.3°C |
Solubility | Soluble in 100 mM in DMSO. |
Vapor Presure | 4.44E-19mmHg at 25°C |
Appearance | White or yellowish powder |
Color | White |
Odor | Odorless |
Merck | 14,10074 |
BRN | 1402009 |
pKa | 12.74±0.70(Predicted) |
Storage Condition | -20°C |
Stability | Stable. Incompatible with strong oxidizing agents. |
Sensitive | Moisture & Light Sensitive |
Refractive Index | 1.6110 (estimate) |
MDL | MFCD00005666 |
Use | X-Gal is a chromogenic substrate for β-galactosidase (β-galactosidase), which produces a blue product under the catalysis of β-galactosidase. |
Hazard Symbols | Xi - Irritant |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S24/25 - Avoid contact with skin and eyes. S37/39 - Wear suitable gloves and eye/face protection S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
WGK Germany | 3 |
FLUKA BRAND F CODES | 8-10-21 |
HS Code | 29389090 |
Hazard Class | IRRITANT |
Reference Show more | 1. Tian Xiaorong, Yu Min, Li Bei, et al. Density-sensing and density-induced quenching of bacterial diversity in biofilm of sediments from Qingdao offshore. 2. [IF = 5.64] Li Jie et al."Investigation of the mineral Acid Bacteria in Kazak Cheese and Their Contributions to Cheese Fermentation." Front Microbiol. 2020 Mar;0:228 |
biological activity | X-GAL (BCIG) is a widely used chromogenic β-galactosidase β-galactosidase substrate, Β-galactosidase cleaves X-gal and produces an insoluble observable blue compound. X-Gal applied to the screening of blue and white spots, the recombinant bacterial colonies with lac genotype were selected. |
Target | Value |
Use | enzyme substrate X-Gal is the chromogenic substrate of β-galactosidase, A blue product is produced under the catalysis of β-galactosidase. It is commonly used for in situ staining detection of β-galactosidase and screening of blue leukoplakia. |